Premium
Cover Picture: Biotechnology Journal 4/2018
Publication year - 2018
Publication title -
biotechnology journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201870041
Subject(s) - endonuclease , ap site , uracil dna glycosylase , ap endonuclease , dna , base excision repair , uracil , chemistry , dna glycosylase , microbiology and biotechnology , computational biology , dna repair , biochemistry , biology
An abasic site generated by uracil DNA glycosylase (UDG, gray pacman) inhibits a nicking endonuclease (silver‐blue pacman) activity that is required to promote an isothermal nicking endonuclease amplification reaction (NEAR), which represents the key concept of our manuscript. In the absence of UDG, a large number of double‐stranded (ds) DNA products (red strand) are generated through NEAR, leading to the high fluorescence signal (left section). However, in the presence of UDG, uracil base (gray ‘U’ in its chemical structure) located at the cleavage site of nicking endonuclease is excised and removed. The resulting abasic site inhibits the nicking endonuclease activity, consequently suppressing NEAR. As a result, few dsDNAs are produced with a negligible fluorescence signal (right section). This is reported by Jun Ki Ahn, Chang Yeol Lee, Ki Soo Park, and Hyun Gyu Park, article number 1700603 .