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Combined Cell Surface Display of β‐ d ‐Glucosidase (BGL), Maltose Transporter (MAL11), and Overexpression of Cytosolic Xylose Reductase (XR) in Saccharomyces cerevisiae Enhance Cellobiose/Xylose Coutilization for Xylitol Bioproduction from Lignocellulosic Biomass
Author(s) -
Guirimand Gregory G. Y.,
Bamba Takahiro,
Matsuda Mami,
Inokuma Kentaro,
Morita Kenta,
Kitada Yuki,
Kobayashi Yuma,
Yukawa Takahiro,
Sasaki Kengo,
Ogino Chiaki,
Hasunuma Tomohisa,
Kondo Akihiko
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201800704
Subject(s) - xylose , xylitol , cellobiose , biochemistry , xylose metabolism , chemistry , xylose isomerase , yeast , lignocellulosic biomass , saccharomyces cerevisiae , metabolic engineering , bioproduction , maltose , fermentation , cellulose , enzyme , cellulase
Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d ‐xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro‐organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae ( S. cerevisiae ) (YPH499‐ Ss XR‐ Aa BGL) expressing cytosolic xylose reductase ( Scheffersomyces stipitis xylose reductase [ Ss XR]), along with a β‐ d ‐glucosidase ( Aspergillus aculeatus β‐glucosidase 1 [ Aa BGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5‐fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter ( Sc MAL11) shows the best performance in xylose transport and is used to generate the strain YPH499‐XR‐ Sc MAL11‐BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae .