Premium
Pichia pastoris as a Versatile Cell Factory for the Production of Industrial Enzymes and Chemicals: Current Status and Future Perspectives
Author(s) -
Zhu Taicheng,
Sun Hongbing,
Wang Meiyu,
Li Yin
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201800694
Subject(s) - pichia pastoris , biochemical engineering , factory (object oriented programming) , protein expression , production (economics) , microbiology and biotechnology , industrial production , computational biology , biology , computer science , biochemistry , engineering , recombinant dna , gene , macroeconomics , keynesian economics , economics , programming language
With nearly three decades of development, Pichia pastoris ( P . pastoris ) has become a powerful eukaryotic protein expression system for the expression of thousands of proteins both on a laboratory and industrial scale. In addition, it has also been extensively used as a cell factory for the production of a variety of chemicals. This review summarizes the bottlenecks and solutions in achieving high‐level secretory protein expression with P. pastoris and then outlines its applications on chemical production with an emphasis on its role as whole‐cell biocatalyst. Furthermore, current challenges and future directions of this important system are also discussed.