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Steps Toward High‐Performance PLA: Economical Production of d ‐Lactate Enabled by a Newly Isolated Sporolactobacillus terrae Strain
Author(s) -
Han Xiao,
Huang Kaiming,
Tang Hongzhi,
Ni Jun,
Liu Jiongqin,
Xu Ping,
Tao Fei
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201800656
Subject(s) - fermentation , strain (injury) , food science , corn steep liquor , raw material , chemistry , biochemistry , biology , microbiology and biotechnology , organic chemistry , anatomy
Optically pure d ‐lactate production has received much attention for its critical role in high‐performance polylactic acid production. However, the current technology can hardly meet the comprehensive demand of industrialization on final titer, productivity, optical purity, and raw material costs. Here, an efficient d ‐lactate producer strain, Sporolactobacillus terrae ( S. terrae ) HKM‐1, is isolated for d ‐lactate production. The strain HKM‐1 shows extremely high d ‐lactate fermentative capability by using peanut meal, soybean meal, or corn steep liquor powder as a sole nitrogen source; the final titers (205.7 g L −1 , 218.9 g L −1 , and 193.9 g L −1 , respectively) and productivities (5.56 g L −1 h −1 , 5.34 g L −1 h −1 , and 3.73 g L −1 h −1 , respectively) of d ‐lactate reached the highest level ever reported. A comparative genomic analysis between S. terrae HKM‐1 and previously reported d ‐lactate high‐producing Sporolactobacillus inulinus ( S. inulinus ) CASD is conducted. The results show that many unrelated genetic features may contribute to the superior performance in d ‐lactate production of S. terrae HKM‐1. This d ‐lactate producer HKM‐1, along with its fermentation process, is promising for sustainable d ‐lactate production by using agro‐industrial wastes.