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Astin C Production by the Endophytic Fungus Cyanodermella asteris in Planktonic and Immobilized Culture Conditions
Author(s) -
Vassaux Antoine,
Tarayre Cédric,
ArguëllesArias Anthony,
Compère Philippe,
Delvigne Frank,
Fickers Patrick,
Jahn Linda,
Lang Alexander,
Leclère Valérie,
LudwigMüller Jutta,
Ongena Marc,
Schafhauser Thomas,
Telek Samuel,
Théatre Ariane,
Berkel Willem J. H.,
Vandenbol Micheline,
Pée KarlHeinz,
Willems Luc,
Wohlleben Wolfgang,
Jacques Philippe
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201800624
Subject(s) - yield (engineering) , liquid culture , fungus , chemistry , biomass (ecology) , spore , botany , biology , chromatography , agronomy , materials science , metallurgy
The fungal endophyte Cyanodermella asteris ( C. asteris ) has been recently isolated from the medicinal plant Aster tataricus ( A. tataricus ). This fungus produces astin C, a cyclic pentapeptide with anticancer and anti‐inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized‐cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre‐inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g −1 and 0.16 mg g −1 respectively).