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Anaerobic C–H Oxyfunctionalization: Coupling of Nitrate Reduction and Quinoline Hydroxylation in Recombinant Pseudomonas putida
Author(s) -
Ütkür Fatma Özde,
Schmid Andreas,
Bühler Bruno
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201800615
Subject(s) - pseudomonas putida , hydroxylation , chemistry , quinoline , nitrate reductase , electron acceptor , denitrification , nitrate , biochemistry , organic chemistry , enzyme , nitrogen
Whole‐cell biocatalysis for C–H oxyfunctionalization depends on and is often limited by O 2 mass transfer. In contrast to oxygenases, molybdenum hydroxylases use water instead of O 2 as an oxygen donor and thus have the potential to relieve O 2 mass transfer limitations. Molybdenum hydroxylases may even allow anaerobic oxyfunctionalization when coupled to anaerobic respiration. To evaluate this option, the coupling of quinoline hydroxylation to denitrification is tested under anaerobic conditions employing Pseudomonas putida ( P. putida ) 86, capable of aerobic growth on quinoline. P. putida 86 reduces both nitrate and nitrite, but at low rates, which does not enable significant growth and quinoline hydroxylation. Introduction of the nitrate reductase from Pseudomonas aeruginosa enables considerable specific quinoline hydroxylation activity (6.9 U g CDW −1 ) under anaerobic conditions with nitrate as an electron acceptor and 2‐hydroxyquinoline as the sole product (further metabolization depends on O 2 ). Hydroxylation‐derived electrons are efficiently directed to nitrate, accounting for 38% of the respiratory activity. This study shows that molybdenum hydroxylase‐based whole‐cell biocatalysts enable completely anaerobic carbon oxyfunctionalization when coupled to alternative respiration schemes such as nitrate respiration.