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Systems Metabolic Engineering of Escherichia coli Improves Coconversion of Lignocellulose‐Derived Sugars
Author(s) -
Kim Joonhoon,
Tremaine Mary,
Grass Jeffrey A.,
Purdy Hugh M.,
Landick Robert,
Kiley Patricia J.,
Reed Jennifer L.
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201800441
Subject(s) - xylose , hydrolysate , lignocellulosic biomass , zymomonas mobilis , metabolic engineering , escherichia coli , sugar , biochemistry , saccharomyces cerevisiae , biofuel , biomass (ecology) , chemistry , food science , xylose metabolism , biology , microbiology and biotechnology , ethanol fuel , fermentation , yeast , gene , hydrolysis , agronomy
Currently, microbial conversion of lignocellulose‐derived glucose and xylose to biofuels is hindered by the fact that most microbes (including Escherichia coli [ E. coli ], Saccharomyces cerevisiae , and Zymomonas mobilis ) preferentially consume glucose first and consume xylose slowly after glucose is depleted in lignocellulosic hydrolysates. In this study, E. coli strains are developed that simultaneously utilize glucose and xylose in lignocellulosic biomass hydrolysate using genome‐scale models and adaptive laboratory evolution. E. coli strains are designed and constructed that coutilize glucose and xylose and adaptively evolve them to improve glucose and xylose utilization. Whole‐genome resequencing of the evolved strains find relevant mutations in metabolic and regulatory genes and the mutations’ involvement in sugar coutilization is investigated. The developed strains show significantly improved coconversion of sugars in lignocellulosic biomass hydrolysates and provide a promising platform for producing next‐generation biofuels.