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Reaction‐Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation
Author(s) -
Isakari Yu,
Kishi Yuhi,
Yoshimoto Noriko,
Yamamoto Shuichi,
Podgornik Aleš
Publication year - 2018
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201700738
Subject(s) - desorption , chemistry , reagent , selectivity , pegylation , macromolecule , chromatography , peg ratio , elution , bovine serum albumin , adsorption , polyethylene glycol , organic chemistry , catalysis , biochemistry , finance , economics
Combining chemical reaction with separation offers several advantages. In this work possibility to induce spontaneous desorption of adsorbed macromolecules, once being PEGylated, through adjustment of the reagent composition is investigated. Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation. BSA solid‐phase PEGylation is performed on Q Sepharose HP. Distribution coefficient of BSA and PEG‐BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model. According to the distribution coefficient the selectivity between BSA and PEG − BSA of around 15 is adjusted by using NaCl. Spontaneous desorption of PEG − BSA is detected with no presence of BSA. However, due to a rather low selectivity, also desorption of BSA occurred at high elution volume. A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption. Selectivity of over 2000 is obtained by proper adjustment of PEG reagent composition. High selectivity enables spontaneous desorption of PEG‐9T without any desorption of activated 9T. Both experiments demonstrates that reaction‐mediated desorption of macromolecules is possible when the reaction conditions are properly tuned.