Premium
Modulator Dynamics Shape the Design Space for Stepwise‐Elution Simulated Moving Bed Chromatographic Separations
Author(s) -
Wayne Chris J.,
Velayudhan Ajoy
Publication year - 2018
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201700664
Subject(s) - dimensionless quantity , elution , simulated moving bed , chromatography , dynamics (music) , space (punctuation) , biological system , chemistry , parameter space , materials science , computer science , physics , mathematics , mechanics , adsorption , organic chemistry , acoustics , biology , operating system , statistics
For proteins and other biological macromolecules, SMB chromatography is best operated non‐isocratically. However, traditional modes of non‐isocratic SMB operation generate significant mobile‐phase modulator dynamics. The mechanisms by which these modulator dynamics affect a separation's success, and thus frame the design space, have yet to be explained quantitatively. Here, the dynamics of the modulator (e.g., salts in ion exchange and hydrophobic interaction chromatography) are explicitly accounted for. This leads to the elucidation of two new design constraints, presented as dimensionless numbers, which quantify the effects of the modulator phenomena and thus predict the success of a non‐isocratic SMB separation. Consequently, these two new design constraints re‐define the SMB design space. Computational and experimental studies at the boundaries of this design space corroborate the theoretical predictions. The design of efficient and robust operating conditions through use of the new design space is also demonstrated.