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An RNA Origami Octahedron with Intrinsic siRNAs for Potent Gene Knockdown
Author(s) -
Høiberg Hans Christian,
Sparvath Steffen M.,
Andersen Veronica L.,
Kjems Jørgen,
Andersen Ebbe S.
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201700634
Subject(s) - small interfering rna , dicer , rna , gene knockdown , trans acting sirna , dna nanotechnology , computational biology , nanomedicine , gene silencing , nucleic acid , nanotechnology , dna origami , biology , dna , microbiology and biotechnology , gene , materials science , genetics , nanoparticle
The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence‐complementarity of nine shorter synthetic strands. High‐yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.