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Progress of CRISPR‐Cas Based Genome Editing in Photosynthetic Microbes
Author(s) -
Naduthodi Mihris Ibnu Saleem,
Barbosa Maria J.,
van der Oost John
Publication year - 2018
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201700591
Subject(s) - crispr , genome editing , genome , toolbox , carbon footprint , synthetic biology , computational biology , biology , biochemical engineering , cyanobacteria , computer science , ecology , gene , greenhouse gas , genetics , bacteria , programming language , engineering
The carbon footprint caused by unsustainable development and its environmental and economic impact has become a major concern in the past few decades. Photosynthetic microbes such as microalgae and cyanobacteria are capable of accumulating value‐added compounds from carbon dioxide, and have been regarded as environmentally friendly alternatives to reduce the usage of fossil fuels, thereby contributing to reducing the carbon footprint. This light‐driven generation of green chemicals and biofuels has triggered the research for metabolic engineering of these photosynthetic microbes. CRISPR‐Cas systems are successfully implemented across a wide range of prokaryotic and eukaryotic species for efficient genome editing. However, the inception of this genome editing tool in microalgal and cyanobacterial species took off rather slowly due to various complications. In this review, we elaborate on the established CRISPR‐Cas based genome editing in various microalgal and cyanobacterial species. The complications associated with CRISPR‐Cas based genome editing in these species are addressed along with possible strategies to overcome these issues. It is anticipated that in the near future this will result in improving and expanding the microalgal and cyanobacterial genome engineering toolbox.