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Three‐Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation
Author(s) -
Gunay Gokhan,
Sever Melike,
Tekinay Ayse B.,
Guler Mustafa O.
Publication year - 2017
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201700080
Subject(s) - laminin , extracellular matrix , microbiology and biotechnology , in vivo , in vitro , cellular differentiation , regenerative medicine , neural stem cell , organoid , biology , chemistry , stem cell , biochemistry , gene
The extracellular matrix (ECM) provides biochemical signals and structural support for cells, and its functional imitation is a fundamental aspect of biomaterial design for regenerative medicine applications. The stimulation of neural differentiation by a laminin protein‐derived epitope in two‐dimensional (2D) and three‐dimensional (3D) environments is investigated. The 3D gel system is found to be superior to its 2D counterpart for the induction of neural differentiation, even in the absence of a crucial biological inducer in nerve growth factor (NGF). In addition, cells cultured in 3D gels exhibits a spherical morphology that is consistent with their form under in vivo conditions. Overall, the present study underlines the impact of bioactivity, dimension, and NGF addition, as well as the cooperative effects thereof, on the neural differentiation of PC‐12 cells. These results underline the significance of 3D culture systems in the development of scaffolds that closely replicate in vivo environments for the formation of cellular organoid models in vitro.