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Compartmentalized metabolic engineering for biochemical and biofuel production
Author(s) -
Huttanus Herbert M.,
Feng Xueyang
Publication year - 2017
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201700052
Subject(s) - compartmentalization (fire protection) , metabolic pathway , metabolic engineering , compartment (ship) , substrate specificity , substrate (aquarium) , biochemical engineering , metabolic network , chemistry , computational biology , biology , biochemistry , microbiology and biotechnology , enzyme , ecology , oceanography , engineering , geology
Sub‐cellular compartments create specialized reaction chambers in eukaryotes. These compartments provide favorable micro‐environments for many metabolic processes. Recently, metabolic engineers have explored the concept of pathway compartmentalization to enhance the performance of metabolic pathways. This strategy offers many unique advantages, including (i) increased local concentrations of enzymes and substrates, (ii) accessing alternate substrate pools, (iii) separation from competing reactions, and (iv) isolation of harmful intermediates or conditions needed for the pathway. In this review, the method of localizing metabolic pathways into specific organelles as well as the benefits of pathway compartmentalization in terms of enhancing the production of value‐added chemicals is discussed.