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Label‐free live cell imaging by Confocal Raman Microscopy identifies CHO host and producer cell lines
Author(s) -
Prats Mateu Batirtze,
Harreither Eva,
Schosserer Markus,
Puxbaum Verena,
Gludovacz Elisabeth,
Borth Nicole,
Gierlinger Notburga,
Grillari Johannes
Publication year - 2017
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201600037
Subject(s) - intracellular , endoplasmic reticulum , cell culture , chinese hamster ovary cell , microbiology and biotechnology , confocal microscopy , confocal , cell , microscopy , biology , raman microspectroscopy , raman spectroscopy , computational biology , chemistry , biochemistry , genetics , pathology , medicine , physics , geometry , mathematics , optics
As a possible viable and non‐invasive method to identify high producing cells, Confocal Raman Microscopy was shown to be able to differentiate CHO host cell lines and derivative production clones. Cluster analysis of spectra and their derivatives was able to differentiate between different producer cell lines and a host, and also distinguished between an intracellular region of high lipid and protein content that in structure resembles the Endoplasmic Reticulum. This ability to identify the ER may be a major contributor to the identification of high producers. PCA enabled the discrimination even of host cell lines and their subclones with inherently higher production capacity. The method is thus a promising option that may contribute to early, non‐invasive identification of high potential candidates during cell line development and possibly could also be used for proof of identity of established production clones.