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Advance in phage display technology for bioanalysis
Author(s) -
Tan Yuyu,
Tian Tian,
Liu Wenli,
Zhu Zhi,
J. Yang Chaoyong
Publication year - 2016
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201500458
Subject(s) - phage display , bioanalysis , computational biology , bacteriophage , lytic cycle , phagemid , nanotechnology , directed evolution , biology , biosensor , peptide library , chemistry , biochemistry , materials science , peptide , gene , escherichia coli , genetics , peptide sequence , virus , mutant
Phage display technology has emerged as a powerful tool for target gene expression and target‐specific ligand selection. It is widely used to screen peptides, proteins and antibodies with the advantages of simplicity, high efficiency and low cost. A variety of targets, including ions, small molecules, inorganic materials, natural and biological polymers, nanostructures, cells, bacteria, and even tissues, have been demonstrated to generate specific binding ligands by phage display. Phages and target‐specific ligands screened by phage display have been widely used as affinity reagents in therapeutics, diagnostics and biosensors. In this review, comparisons of different types of phage display systems are first presented. Particularly, microfluidic‐based phage display, which enables screening with high throughput, high efficiency and integration, is highlighted. More importantly, we emphasize the advances in biosensors based on phages or phage‐derived probes, including nonlytic phages, lytic phages, peptides or proteins screened by phage display, phage assemblies and phage‐nanomaterial complexes. However, more efficient and higher throughput phage display methods are still needed to meet an explosion in demand for bioanalysis. Furthermore, screening of cyclic peptides and functional peptides will be the hotspot in bioanalysis.