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Microalgal bioengineering for sustainable energy development: Recent transgenesis and metabolic engineering strategies
Author(s) -
Banerjee Chiranjib,
Singh Puneet Kumar,
Shukla Pratyoosh
Publication year - 2016
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201500284
Subject(s) - metabolic engineering , biochemical engineering , synthetic biology , microbiology and biotechnology , in silico , biomedicine , metabolic flux analysis , computational biology , metabolomics , chlamydomonas reinhardtii , computer science , biology , engineering , bioinformatics , genetics , metabolism , gene , endocrinology , mutant
Exploring the efficiency of algae to produce remarkable products can be directly benefitted by studying its mechanism at systems level. Recent advents in biotechnology like flux balance analysis (FBA), genomics and in silico proteomics minimize the wet lab exertion. It is understood that FBA predicts the metabolic products, metabolic pathways and alternative pathway to maximize the desired product, and these are key components for microalgae bio‐engineering. This review encompasses recent transgenesis techniques and metabolic engineering strategies applied to different microalgae for improving different traits. Further it also throws light on RNAi and riboswitch engineering based methods which may be advantageous for high throughput microalgal research. A valid and optimally designed microalga can be developed where every engineering strategies meet each other successfully and will definitely fulfill the market needs. It is also to be noted that Omics (viz. genetic and metabolic manipulation with bioinformatics) should be integrated to develop a strain which could prove to be a futuristic solution for sustainable development for energy.