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Patterning hypoxic multicellular spheroids in a 3D matrix – a promising method for anti‐tumor drug screening
Author(s) -
Ma Jingyun,
Zhang Xu,
Liu Yang,
Yu Haibo,
Liu Lianqing,
Shi Yang,
Li Yanfeng,
Qin Jianhua
Publication year - 2016
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201500183
Subject(s) - spheroid , multicellular organism , tumor microenvironment , glioma , 3d cell culture , microbiology and biotechnology , biology , cancer research , angiogenesis , in vitro , cell culture , extracellular matrix , cell , tumor cells , biochemistry , genetics
3D multicellular spheroid models are of great value in the investigation of tumor biology and tumor responses to chemotherapy and radiation. To establish a mimicking tumor microenvironment in vitro, we developed a straightforward method by patterning hypoxic multicellular spheroids in a 3D matrix. The efficacy of this approach was evaluated by characterizing spheroid formation, invasive capability and phenotypic transition in aggressive human glioma cells. We observed enhanced cell proliferation, spheroid formation and invasive capability in U87 glioma cells transfected with hypoxia‐inducible factors (HIFs) compared with non‐treated cells. We also demonstrated that the overexpression of HIFs in hypoxic glioma cells may promote cell migration by epithelial‐mesenchymal transition within the 3D matrix. Compared with conventional 3D culturing techniques, the simple operation, rapid prototyping, low cost and high throughput format of the micro‐patterning method facilitates the characterization of cell proliferation, migration, phenotypic function and drug evaluation in physiologically relevant 3D microenvironments. This in vitro 3D system can recapitulate the physiologically relevant tumor microenvironment and is a promising method for 3D anti‐tumor drug screening and the identification of novel targets for tumor invasion and angiogenesis.