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Prediction of reversible IgG1 aggregation occurring in a size exclusion chromatography column is enabled through a model based approach
Author(s) -
Ojala Frida,
Sellberg Anton,
Hansen Thomas Budde,
Hansen Ernst Broberg,
Nilsson Bernt
Publication year - 2015
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201500160
Subject(s) - size exclusion chromatography , chromatography , column (typography) , chemistry , computer science , biochemistry , telecommunications , frame (networking) , enzyme
One important aspect of antibody separation being studied today is aggregation, as this not only leads to a loss in yield, but aggregates can also be hazardous if injected into the body. The aim of this study was to determine whether the methodology applied in the previous study could be used to predict the aggregation of a different batch of IgG1, and to model the aggregation occurring in a SEC column. Aggregation was found to be reversible. The equilibrium parameter was found to be 272 M ‐1 and the reaction kinetic parameter 1.33 × 10 ‐5 s ‐1 , both within the 95% confidence interval of the results obtained in the previous work. The effective diffusivities were estimated to be 1.45 × 10 ‐13 and 1.90 10 ‐14 m 2 /s for the monomers and dimers, respectively. Good agreement was found between the new model and the chromatograms obtained in the SEC experiments. The model was also able to predict the decrease of dimers due to the dilution and separation in the SEC column during long retention times.