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Phenolic compounds: Strong inhibitors derived from lignocellulosic hydrolysate for 2,3‐butanediol production by Enterobacter aerogenes
Author(s) -
Lee Sang Jun,
Lee Ju Hun,
Yang Xiaoguang,
Kim Sung Bong,
Lee Ja Hyun,
Yoo Hah Young,
Park Chulhwan,
Kim Seung Wook
Publication year - 2015
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201500090
Subject(s) - lignocellulosic biomass , 2,3 butanediol , hydrolysate , chemistry , biorefinery , enterobacter aerogenes , biomass (ecology) , food science , enzymatic hydrolysis , fermentation , butanediol , hydrolysis , raw material , organic chemistry , biochemistry , biology , agronomy , escherichia coli , gene
Lignocellulosic biomass are attractive feedstocks for 2,3‐butanediol production due to their abundant supply and low price. During the hydrolysis of lignocellulosic biomass, various byproducts are formed and their effects on 2,3‐butanediol production were not sufficiently studied compared to ethanol production. Therefore, the effects of compounds derived from lignocellulosic biomass (weak acids, furan derivatives and phenolics) on the cell growth, the 2,3‐butanediol production and the enzymes activity involved in 2,3‐butanediol production were evaluated using Enterobacter aerogenes ATCC 29007. The phenolic compounds showed the most toxic effects on cell growth, 2,3‐butanediol production and enzyme activity, followed by furan derivatives and weak acids. The significant effects were not observed in the presence of acetic acid and formic acid. Also, feasibility of 2,3‐butanediol production from lignocellulosic biomass was evaluated using Miscanthus as a feedstock. In the fermentation of Miscanthus hydrolysate, 11.00 g/L of 2,3‐butanediol was obtained from 34.62 g/L of reducing sugar. However, 2,3‐butanediol was not produced when the concentration of total phenolic compounds in the hydrolysate increased to more than 1.5 g/L. The present study provides useful information to develop strategies for biological production of 2,3‐butanediol and to establish biorefinery for biochemicals from lignocellulosic biomass.