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Dual utilization of NADPH and NADH cofactors enhances xylitol production in engineered Saccharomyces cerevisiae
Author(s) -
Jo JungHyun,
Oh SunYoung,
Lee HyeunSoo,
Park YongCheol,
Seo JinHo
Publication year - 2015
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201500068
Subject(s) - xylitol , xylose , saccharomyces cerevisiae , biochemistry , cofactor , fermentation , yeast , metabolic engineering , chemistry , enzyme
Xylitol, a natural sweetener, can be produced by hydrogenation of xylose in hemicelluloses. In microbial processes, utilization of only NADPH cofactor limited commercialization of xylitol biosynthesis. To overcome this drawback, Saccharomyces cerevisiae D452‐2 was engineered to express two types of xylose reductase (XR) with either NADPH‐dependence or NADH‐preference. Engineered S. cerevisiae DWM expressing both the XRs exhibited higher xylitol productivity than the yeast strain expressing NADPH‐dependent XR only (DWW) in both batch and glucose‐limited fed‐batch cultures. Furthermore, the coexpression of S. cerevisiae ZWF1 and ACS1 genes in the DWM strain increased intracellular concentrations of NADPH and NADH and improved maximum xylitol productivity by 17%, relative to that for the DWM strain. Finally, the optimized fed‐batch fermentation of S. cerevisiae DWM‐ZWF1‐ACS1 resulted in 196.2 g/L xylitol concentration, 4.27 g/L h productivity and almost the theoretical yield. Expression of the two types of XR utilizing both NADPH and NADH is a promising strategy to meet the industrial demands for microbial xylitol production.