Premium
Chassis organism from Corynebacterium glutamicum : The way towards biotechnological domestication of Corynebacteria
Author(s) -
de Lorenzo Víctor
Publication year - 2015
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201400493
Subject(s) - corynebacterium glutamicum , synthetic biology , biology , organism , microbiology and biotechnology , computational biology , biochemical engineering , chassis , computer science , bacteria , genetics , engineering , structural engineering
For a long time, Corynebacteria have been the organisms of choice for industrial bioproduction of amino acids. Later on, Corynebacteria have also been used for making biofuels and a suite of added-value chemicals. In this issue of Biotechnology Journal, Unthan et al. provide a splendid example of how systems and synthetic biology approaches are instrumental for significantly increasing the value of Corynebacterium glutamicum as a platform strain for industrial applications. To this end, genomic segments that appeared to be non-essential for maintaining every desirable trait in C. glutamicum were delineated. A massive, recursive deletion of each (or most) of such apparently useless DNA was then carried out to verify the relevance of the excised sequences, and the resulting C. glutamicum variants were tested under various\udgrowth conditions. This approach produced not only interesting new strains but also raised new questions on how to design reliable microbial chassis that fulfil the biotechnological promise of synthetic biology.Peer reviewe