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Designing cell lines for viral vaccine production: Where do we stand?
Author(s) -
Genzel Yvonne
Publication year - 2015
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201400388
Subject(s) - chinese hamster ovary cell , cell culture , microcarrier , bioreactor , cell , microbiology and biotechnology , bioprocess , biology , primary cell , embryonic stem cell , chemistry , biochemistry , genetics , paleontology , botany , gene
Established animal cells, such as Vero, Madin Darby canine kidney (MDCK) or chicken embryo fibroblasts (CEFs), are still the main cell lines used for viral vaccine production, although new “designer cells” have been available for some years. These designer cell lines were specifically developed as a cell substrate for one application and are well characterized. Later screening for other possible applications widened the product range. These cells grow in suspension in chemically defined media under controlled conditions and can be used for up to 100 passages. Scale‐up is easier and current process options allow cultivation in disposable bioreactors at cell concentrations higher than 1×10 7 cells/mL. This review covers the limitations of established cell lines and discusses the requirements and screening options for new host cells. Currently available designer cells for viral vaccine production (PER.C6, CAP, AGE1.CR, EB66 cells), together with other new cell lines (PBS‐1, QOR/2E11, SogE, MFF‐8C1 cells) that were recently described as possible cell substrates are presented. Using current process knowledge and cell line development tools, future upstream processing could resemble today's Chinese hamster ovary (CHO) cell processes for monoclonal antibody production: small scale bioreactors (disposable) in perfusion or fed‐batch mode with cell concentrations above 1×10 8 cells/mL.