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Universal probe amplification: Multiplex screening technologies for genetic variations
Author(s) -
Park Jung Hun,
Park Ki Soo,
Lee Kyungmee,
Jang Hyowon,
Park Hyun Gyu
Publication year - 2015
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201400219
Subject(s) - multiplex , computational biology , biology , multiplex polymerase chain reaction , genetics , multiplex ligation dependent probe amplification , identification (biology) , molecular diagnostics , polymerase chain reaction , gene , botany , exon
In order to achieve multiplex screening of genetic variations, multiplex amplification of target genomic DNA is necessary. Universal amplification technology meets this requirement by simultaneously amplifying a number of different regions within the target genomic DNA using a single pair of universal primers and thus eliminating the limitations associated with the use of multiple pairs of primers. We comprehensively review universal probe amplification and its use with multiplex technologies for the identification of the most representative genetic variation, i. e. single nucleotide polymorphisms. The progress and key issues relating to universal probe amplification are discussed and the representative technologies are summarized with an emphasis on their application for the identification of susceptibility to human diseases.