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High‐level conversion of L ‐lysine into 5‐aminovalerate that can be used for nylon 6,5 synthesis
Author(s) -
Park Si Jae,
Oh Young Hoon,
Noh Won,
Kim Hye Young,
Shin Jae Ho,
Lee Eun Gyo,
Lee Seungwoon,
David Yokimiko,
Baylon Mary Grace,
Song Bong Keun,
Jegal Jonggeon,
Lee Sang Yup,
Lee Seung Hwan
Publication year - 2014
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201400156
Subject(s) - lysine , yield (engineering) , escherichia coli , pseudomonas putida , chemistry , monomer , polymerization , nuclear chemistry , materials science , biochemistry , organic chemistry , amino acid , polymer , enzyme , metallurgy , gene
Abstract L ‐Lysine is a potential feedstock for the production of bio‐based precursors for engineering plastics. In this study, we developed a microbial process for high‐level conversion of L ‐lysine into 5‐aminovalerate (5AVA) that can be used as a monomer in nylon 6,5 synthesis. Recombinant Escherichia coli WL3110 strain expressing Pseudomonas putida delta‐aminovaleramidase (DavA) and lysine 2‐monooxygenase (DavB) was grown to high density in fed‐batch culture and used as a whole cell catalyst. High‐density E. coli WL3110 expressing DavAB, grown to an optical density at 600 nm (OD 600 ) of 30, yielded 36.51 g/L 5AVA from 60 g/L L ‐lysine in 24 h. Doubling the cell density of E. coli WL3110 improved the conversion yield to 47.96 g/L 5AVA from 60 g/L of L ‐lysine in 24 h. 5AVA production was further improved by doubling the L ‐lysine concentration from 60 to 120 g/L. The highest 5AVA titer (90.59 g/L; molar yield 0.942) was obtained from 120 g/L L ‐lysine by E. coli WL3110 cells grown to OD 600 of 60. Finally, nylon 6,5 was synthesized by bulk polymerization of ϵ‐caprolactam and δ‐valerolactam prepared from microbially synthesized 5AVA. The hybrid system demonstrated here has promising possibilities for application in the development of industrial bio‐nylon production processes.