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Silencing the radicals improves Click Chemistry
Author(s) -
Tournebize Régis,
Dragavon Joe,
Miller Andrew D.,
Shorte Spencer
Publication year - 2014
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201400103
Subject(s) - fluorophore , fluorescence , click chemistry , chemistry , förster resonance energy transfer , biophysics , two photon excitation microscopy , excited state , photochemistry , nanotechnology , combinatorial chemistry , materials science , biology , optics , physics , nuclear physics
Modern fluorescence imaging microscopy in living and fixed material makes use of fluorescent probes to label targeted entities. Common labelling approaches include classical immunocytochemistry, expression of chimerically tagged fluorescent protein domains, and chemical affinity-binding or covalent labelling. Of these methods, the so-called "Click Chemistry", is emerging as one of the most influential labelling chemistries introduced in recent times, offering enormous utility for bio-orthoganol attachment of fluorescent probes to biological target entities. In this issue of Biotechnology Journal, Löschberger, Niehörster and Sauer report "ClickOx", a Click Chemistry protocol that uses an enzymatic oxygen scavenger system to reduce concurrent ROS-associated damage during Click labeling.