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Biologically synthesized silver nanoparticles induce neuronal differentiation of SH‐SY5Y cells via modulation of reactive oxygen species, phosphatases, and kinase signaling pathways
Author(s) -
Dayem Ahmed Abdal,
Kim BongWoo,
Gurunathan Sangiliyandi,
Choi Hye Yeon,
Yang Gwangmo,
Saha Subbroto Kumar,
Han Dawoon,
Han Jihae,
Kim Kyeongseok,
Kim JinHoi,
Cho SsangGoo
Publication year - 2014
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201300555
Subject(s) - sh sy5y , neurite , microbiology and biotechnology , neurogenesis , mapk/erk pathway , cellular differentiation , reactive oxygen species , intracellular , kinase , protein kinase b , phosphatase , biology , signal transduction , chemistry , in vitro , cell culture , biochemistry , neuroblastoma , phosphorylation , genetics , gene
Nano‐scale materials are noted for unique properties, distinct from those of their bulk material equivalents. In this study, we prepared spherical silver nanoparticles (AgNPs) with an average size of about 30 nm and tested their potency to induce neuronal differentiation of SH‐SY5Y cells. Human neuroblastoma SH‐SY5Y cells are considered an ideal in vitro model for studying neurogenesis, as they can be maintained in an undifferentiated state or be induced to differentiate into neuron‐like phenotypes in vitro by several differentiation‐inducing agents. Treatment of SH‐SY5Y cells by biologically synthesized AgNPs led to cell morphological changes and significant increase in neurite length and enhanced the expression of neuronal differentiation markers such as Map‐2, β‐tubulin III, synaptophysin, neurogenin‐1, Gap‐43, and Drd‐2. Furthermore, we observed an increase in generation of intracellular reactive oxygen species (ROS), activation of several kinases such as ERK and AKT, and downregulation of expression of dual‐specificity phosphatases (DUSPs) in AgNPs‐exposed SH‐SY5Y cells. Our results suggest that AgNPs modulate the intracellular signaling pathways, leading to neuronal differentiation, and could be applied as promising nanomaterials for stem cell research and therapy. same article under 10.1002/biot.201400555 .