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S‐Fms signalobody enhances myeloid cell growth and migration
Author(s) -
Kawahara Masahiro,
Hitomi Azusa,
Nagamune Teruyuki
Publication year - 2014
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201300346
Subject(s) - microbiology and biotechnology , receptor tyrosine kinase , protein kinase b , mapk/erk pathway , cell migration , progenitor cell , cell growth , pi3k/akt/mtor pathway , signal transduction , biology , cell , chemistry , stem cell , biochemistry
Since receptor tyrosine kinases (RTKs) control various cell fates in many types of cells, mimicry of RTK functions is promising for artificial control of cell fates. We have previously developed single‐chain Fv (scFv)/receptor chimeras named signalobodies that can mimic receptor signaling in response to a specific antigen. While the RTK‐based signalobodies enabled us to control cell growth and migration, further extension of applicability in another cell type would underlie the impact of the RTK‐based signalobodies. In this study, we applied the scFv‐c‐Fms (S‐Fms) signalobody in a murine myeloid progenitor cell line, FDC‐P1. S‐Fms transduced a fluorescein‐conjugated BSA (BSA‐FL)‐dependent growth signal and activated downstream signaling molecules including MEK, ERK, Akt, and STAT3, which are major constituents of Ras/MAPK, PI3K/Akt, and JAK/STAT signaling pathways. In addition, S‐Fms transduced a migration signal as demonstrated by the transwell‐based migration assay. Direct real‐time observation of the cells further confirmed that FDC/S‐Fms cells underwent directional cell migration toward a positive gradient of BSA‐FL. These results demonstrated the utility of the S‐Fms signalobody for controlling growth and migration of myeloid cells. Further extension of our approach includes economical large‐scale production of practically relevant blood cells as well as artificial control of cell migration for tissue regeneration and immune response.