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Acri‐2,7‐Py, a bright red‐emitting DNA probe identified through screening of a distyryl dye library
Author(s) -
NaudMartin Delphine,
MartinBenlloch Xavier,
Poyer Florent,
MahuteauBetzer Florence,
TeuladeFichou MariePaule
Publication year - 2014
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201300197
Subject(s) - nucleic acid , dna , photobleaching , fluorescence , chemistry , microbiology and biotechnology , oligonucleotide , biophysics , stain , combinatorial chemistry , staining , biochemistry , biology , genetics , optics , physics
The identification of DNA sensors is still a challenge since no DNA probe possesses all the photophysical properties required for live‐cell imaging: high fluorescence yield, red emission, permeability, no photobleaching and no cytotoxicity. We describe the preparation of a distyryl dye library and its evaluation on a panel of nucleic acids with various structures (duplex DNA, quadruplex DNA and RNA). The screening involved measuring the modification of the fluorescence properties of the dyes with or without nucleic acids on a microplate reader, and allowed the identification of selective quadruplex DNA ligands with good affinities. Using this screening method we discovered a new bright red‐emitting DNA stain, Acri‐2,7‐Py, for fixed cells. In living cells, the staining was not nuclear and photodamage generated through illumination induced cellular death. These processes require further studies to determine the relevance of Acri‐2,7‐Py in photodynamic therapy.