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Efficient recovery of recombinant proteins from cereal endosperm is affected by interaction with endogenous storage proteins
Author(s) -
Peters Jenny,
Sabalza Maite,
Ramessar Koreen,
Christou Paul,
Capell Teresa,
Stöger Eva,
Arcalís Elsa
Publication year - 2013
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201300068
Subject(s) - endosperm , prolamin , recombinant dna , storage protein , endogeny , biochemistry , antibody , chemistry , in vitro , biology , microbiology and biotechnology , gene , immunology
Cereal seeds are versatile platforms for the production of recombinant proteins because they provide a stable environment for protein accumulation. Endogenous seed storage proteins, however, include several prolamin‐type polypeptides that aggregate and crosslink via intermolecular disulfide bridges, which could potentially interact with multimeric recombinant proteins such as antibodies, which assemble in the same manner. We investigated this possibility by sequentially extracting a human antibody expressed in maize endosperm, followed by precipitation in vitro with zein. We provide evidence that a significant proportion of the antibody pool interacts with zein and therefore cannot be extracted using non‐reducing buffers. Immunolocalization experiments demonstrated that antibodies targeted for secretion were instead retained within zein bodies because of such covalent interactions. Our findings suggest that the production of soluble recombinant antibodies in maize could be enhanced by eliminating or minimizing interactions with endogenous storage proteins.