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Toward systems metabolic engineering of Aspergillus and Pichia species for the production of chemicals and biofuels
Author(s) -
Caspeta Luis,
Nielsen Jens
Publication year - 2013
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201200345
Subject(s) - metabolic engineering , systems biology , aspergillus , biology , computational biology , synthetic biology , microbiology and biotechnology , genomics , biochemical engineering , genome , genetics , gene , engineering
Recently genome sequence data have become available for Aspergillus and Pichia species of industrial interest. This has stimulated the use of systems biology approaches for large‐scale analysis of the molecular and metabolic responses of Aspergillus and Pichia under defined conditions, which has resulted in much new biological information. Case‐specific contextualization of this information has been performed using comparative and functional genomic tools. Genomics data are also the basis for constructing genome‐scale metabolic models, and these models have helped in the contextualization of knowledge on the fundamental biology of Aspergillus and Pichia species. Furthermore, with the availability of these models, the engineering of Aspergillus and Pichia is moving from traditional approaches, such as random mutagenesis, to a systems metabolic engineering approach. Here we review the recent trends in systems biology of Aspergillus and Pichia species, highlighting the relevance of these developments for systems metabolic engineering of these organisms for the production of hydrolytic enzymes, biofuels and chemicals from biomass.