Premium
Protein design in systems metabolic engineering for industrial strain development
Author(s) -
Chen Zhen,
Zeng AnPing
Publication year - 2013
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201200238
Subject(s) - metabolic engineering , synthetic biology , systems biology , context (archaeology) , protein engineering , metabolic pathway , biochemical engineering , protein expression , computer science , computational biology , biology , engineering , metabolism , biochemistry , enzyme , paleontology , gene
Accelerating the process of industrial bacterial host strain development, aimed at increasing productivity, generating new bio‐products or utilizing alternative feedstocks, requires the integration of complementary approaches to manipulate cellular metabolism and regulatory networks. Systems metabolic engineering extends the concept of classical metabolic engineering to the systems level by incorporating the techniques used in systems biology and synthetic biology, and offers a framework for the development of the next generation of industrial strains. As one of the most useful tools of systems metabolic engineering, protein design allows us to design and optimize cellular metabolism at a molecular level. Here, we review the current strategies of protein design for engineering cellular synthetic pathways, metabolic control systems and signaling pathways, and highlight the challenges of this subfield within the context of systems metabolic engineering.