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A thermally baffled device for highly stabilized convective PCR
Author(s) -
Chang HsiaoFen Grace,
Tsai YunLong,
Tsai ChuanFu,
Lin ChingKo,
Lee PeiYu,
Teng PingHua,
Su Chen,
Jeng ChienChung
Publication year - 2012
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201100453
Subject(s) - convection , materials science , chemistry , mechanics , physics
Rayleigh‐Bénard convective PCR is a simple and effective design for amplification of DNA. Convective PCR is, however, extremely sensitive to environmental temperature fluctuations, especially when using small‐ diameter test tubes. Therefore, this method is inherently unstable with limited applications. Here, we present a convective PCR device that has been modified by adding thermal baffles. With this thermally baffled device the influence from fluctuations in environmental temperature were significantly reduced, even in a wind tunnel (1 m/s). The thermally baffled PCR instrument described here has the potential to be used as a low‐cost, point‐of‐care device for PCR‐based molecular diagnostics in the field.