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An energy‐saving glutathione production method from low‐temperature cooked rice using amylase‐expressing Saccharomyces cerevisiae
Author(s) -
Hara Kiyotaka Y.,
Kim Songhee,
Kiriyama Kentaro,
Yoshida Hideyo,
Arai Shogo,
Ishii Jun,
Ogino Chiaki,
Fukuda Hideki,
Kondo Akihiko
Publication year - 2012
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201100432
Subject(s) - fermentation , saccharomyces cerevisiae , glutathione , starch , yeast , food science , chemistry , amylase , tripeptide , hydrolysis , biochemistry , enzyme , peptide
Glutathione is a valuable tripeptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is industrially produced by fermentation using Saccharomyces cerevisiae . Before the glutathione fermentation process with S. cerevisiae , a glucose extraction process from starchy materials is required. This glucose extraction is usually carried out by converting starchy materials to starch using high‐temperature cooking and subsequent hydrolysis by amylases to convert starch to glucose. In this study, to develop an energy‐saving glutathione production process by reducing energy consumption during the cooking step, we efficiently produced glutathione from low‐temperature cooked rice using amylase‐expressing S. cerevisiae . The combination of the amylase‐expressing yeast with low‐temperature cooking is potentially applicable to a variety of energy‐saving bio‐production methods of chemicals from starchy bio‐resources.