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Tools to evaluate the conformation of protein products
Author(s) -
Manta Bruno,
Obal Gonzalo,
Ricciardi Alejandro,
Pritsch Otto,
Denicola Ana
Publication year - 2011
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201100107
Subject(s) - recombinant dna , computational biology , protein structure , chemistry , protein engineering , protein function , monoclonal antibody , sequence (biology) , biochemistry , biology , antibody , enzyme , gene , immunology
Production of recombinant proteins is a process intensively used in the research laboratory. In addition, the main biotechnology market products are recombinant proteins and monoclonal antibodies. The biological (and clinical) properties of the protein product strongly depend on the conformation of the polypeptide. Therefore, assessment of the correct conformation of the produced protein is crucial. There is no single method to assess every aspect of protein structure or function. Depending on the protein, the methods of choice vary. There are general methods to evaluate not only mass and primary sequence of the protein, but also higher‐order structure. This review outlines the principal techniques for determining the conformation of a protein from structural (biophysical methods) to functional (in vitro binding assays) analyses.