Premium
Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform
Author(s) -
Zhang Yan,
Jia Wenjuan,
Cui Miao,
Dong Chuan,
Shuang Shaomin,
Kwan Yuen,
Choi Martin M. F.
Publication year - 2011
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201000395
Subject(s) - biosensor , eggshell membrane , glucose oxidase , colloidal gold , nanoparticle , repeatability , chemistry , nanotechnology , detection limit , chromatography , membrane , materials science , biochemistry
A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GO x ) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GO x on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5–525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% ( n = 6), good operational stability with over 300 measurements and long‐term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost‐effective biosensors for biotechnological, biomedical and industrial use.