Premium
Gonadotropin purification from horse serum applying magnetic beads
Author(s) -
Müller Christine,
PreußerKunze Andrea,
Wagner Kerstin,
Franzreb Matthias
Publication year - 2011
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201000380
Subject(s) - chromatography , equine chorionic gonadotropin , glycoprotein , fractionation , horse , chemistry , biology , hormone , biochemistry , paleontology , ovulation
The glycoprotein hormone equine chorionic gonadotropin (eCG) is a commercial product used in animal breeding as well as in veterinary medicine. The current state of the art for the purification of eCG from serum is pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed‐bed chromatography. Two simplified processes, including the use of magnetic microsorbents for the purification of eCG have been developed. The processes reduce or even omit the use of organic solvents and the required solid‐liquid separation steps, thus making them potential candidates for a first commercial application of magnetic beads in bioprocessing.