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One‐step enzyme extraction and immobilization for biocatalysis applications
Author(s) -
Cassimjee Karim Engelmark,
Kourist Robert,
Lindberg Diana,
Wittrup Larsen Marianne,
Thanh Nguyen Hong,
Widersten Mikael,
Bornscheuer Uwe T.,
Berglund Per
Publication year - 2011
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201000357
Subject(s) - chemistry , immobilized enzyme , biocatalysis , candida antarctica , lipase , bacillus subtilis , pectinase , extraction (chemistry) , chromatography , esterase , hydrolysis , organic chemistry , enzyme , catalysis , biology , ionic liquid , bacteria , genetics
An extraction/immobilization method for HIs 6 ‐tagged enzymes for use in synthesis applications is presented. By modifying silica oxide beads to be able to accommodate metal ions, the enzyme was tethered to the beads after adsorption of Co(II). The beads were successfully used for direct extraction of C. antarctica lipase B (CalB) from a periplasmic preparation with a minimum of 58% activity yield, creating a quick one‐step extraction‐immobilization protocol. This method, named HisSi Immobilization, was evaluated with five different enzymes [ Candida antarctica lipase B (CalB), Bacillus subtilis lipase A (BslA), Bacillus subtilis esterase (BS2), Pseudomonas fluorescence esterase (PFE), and Solanum tuberosum epoxide hydrolase 1 (StEH1)]. Immobilized CalB was effectively employed in organic solvent (cyclohexane and acetonitrile) in a transacylation reaction and in aqueous buffer for ester hydrolysis. For the remaining enzymes some activity in organic solvent could be shown, whereas the non‐immobilized enzymes were found inactive. The protocol presented in this work provides a facile immobilization method by utilization of the common His 6 ‐tag, offering specific and defined means of binding a protein in a specific location, which is applicable for a wide range of enzymes.