Automated lab‐scale production of PVA/PEG‐enzyme immobilisates | Zendy

Bieler Nora C. | Zendy; AnsorgeSchumacher Marion B. | Zendy; Greiner Lasse | Zendy

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Automated lab‐scale production of PVA/PEG‐enzyme immobilisates

Author(s) -

Bieler Nora C.,

AnsorgeSchumacher Marion B.,

Greiner Lasse

Publication year - 2010

Publication title -

biotechnology journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.144

H-Index - 84

eISSN - 1860-7314

pISSN - 1860-6768

DOI - 10.1002/biot.201000070

Subject(s) - biocatalysis , nozzle , peg ratio , process engineering , injector , scale up , coacervate , microreactor , glyoxal , materials science , nanotechnology , chemistry , chemical engineering , biochemical engineering , computer science , chromatography , mechanical engineering , engineering , organic chemistry , catalysis , physics , ionic liquid , finance , classical mechanics , economics

Entrapment of biocatalysts by cryogelation is a gentle method to extend the scope of biocatalysis. To foster the use of this versatile method we devised an automated injector for the production of PVA/PEG beads. The device consists of a thermostated reservoir connected to a programmable injector nozzle and an agitated receiving bath for the droplets. This lab‐scale production unit yields up to 1500 beads with immobilized enzyme per minute with a narrow size distribution and good roundness.

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