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Anti‐inflammatory and enzyme inhibitory activities of a crude extract and a pterocarpan isolated from the aerial parts of Vitex agnus‐castus
Author(s) -
Ahmad Bashir,
Azam Sadiq,
Bashir Shumaila,
Khan Ibrar,
Adhikari Achyut,
Choudhary Muhammad Iqbal
Publication year - 2010
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201000020
Subject(s) - chemistry , ethyl acetate , antibacterial activity , traditional medicine , minimum inhibitory concentration , fraction (chemistry) , hemagglutination , chromatography , in vitro , bacteria , biochemistry , biology , medicine , genetics , antigen
Abstract A new compound, 6a,11a‐dihydro‐6H‐[1] benzofuro [3,2‐c][1,3]dioxolo[4,5‐g]chromen‐9‐ol was isolated from the ethyl acetate fraction of Vitex agnus‐castus . The structure of this compound was identified with the help of spectroscopic techniques ( 13 C NMR, 1 H NMR, HMBC, HMQC, NOESY and COSY). The compound showed low urease‐ (32.0%) and chymotrypsin‐ (31.4%) inhibitory activity, and moderate (41.3%) anti‐inflammatory activity. The crude extract and various fractions obtained from the aerial parts of the plant were also screened for possible in vitro hemagglutination, antibacterial and phytotoxic activities. No hemagglutination activity against human erythrocytes was observed in crude extracts and fractions of V. agnus‐castus . The fractions and crude methanolic extract showed moderate and low antibacterial activity. Exceptions were the CHCl 3 fraction, which showed significant antibacterial activity against Klebsiella pneumonia (81% with MIC 50 =2.19 mg/mL), the n ‐hexane fraction, which exhibited no activity against Salmonella typhi , and the CHCl 3 and aqueous fractions, which showed no activity against Bacillus pumalis . Moderate phytotoxic activity (62.5%) was observed by n ‐hexane fraction of V. agnus‐castus against Lemna minor L at 1000 μg/mL.