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Liposomes destabilize tRNA during heat stress
Author(s) -
Suga Keishi,
Umakoshi Hiroshi,
Tomita Hibiki,
Tanabe Tomoyuki,
Shimanouchi Toshinori,
Kuboi Ryoichi
Publication year - 2010
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200900289
Subject(s) - liposome , transfer rna , chemistry , heat stress , biology , biochemistry , rna , zoology , gene
Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T m ) of tRNA was calculated from normalized θ 208 . Addition of POPC/Ch liposomes decreased the T m value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.