Premium
Magnetizable antibody‐like proteins
Author(s) -
Dehal Prabhjyot Kaur,
Livingston Christine Frances,
Dunn Claire Geekie,
Buick Richard,
Luxton Richard,
Pritchard David John
Publication year - 2010
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200900273
Subject(s) - ferritin , fusion protein , fusion , chemistry , paramagnetism , iron binding proteins , biophysics , antigen , antibody , molecule , biochemistry , biology , physics , organic chemistry , linguistics , philosophy , genetics , quantum mechanics , immunology , gene , recombinant dna
Use of paramagnetic particles to isolate molecules or cells from complex media is well established. Typically, particles are manufactured and coated with a biological molecule that confers specific biorecognition. Incubation of particles with sample and exposure to magnetic fields isolates the species of interest. We have designed, produced and assessed magnetized fusion proteins consisting of the antigen‐binding portion of an antibody (single chain variable fraction; scFv) fused to the heavy chain of the iron‐binding protein ferritin. The fusion protein subunits expressed in E. coli assemble to form a fusion protein consisting of a ferritin sphere with scFvs on the surface. The fusion proteins were chemically magnetized by introducing a paramagnetic iron core. The resultant fusion protein was shown to be magnetizable and capable of binding target antigens. These “organic” magnetizable particles possess a number of theoretical advantages over traditional inorganic particles.