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Recent trends in non‐viral vector‐mediated gene delivery
Author(s) -
Pathak Atul,
Patnaik Soma,
Gupta Kailash Chand
Publication year - 2009
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200900161
Subject(s) - nanomedicine , electroporation , nucleic acid , gene delivery , oligonucleotide , viral vector , genetic enhancement , computational biology , liposome , vector (molecular biology) , dna , biology , nanotechnology , gene , microbiology and biotechnology , nanoparticle , biochemistry , materials science , recombinant dna
Abstract Nucleic acids‐based next generation biopharmaceuticals ( i.e. , pDNA, oligonucleotides, short interfering RNA) are potential pioneering materials to cope with various incurable diseases. However, several biological barriers present a challenge for efficient gene delivery. On the other hand, developments in nanotechnology now offer numerous non‐viral vectors that have been fabricated and found capable of transmitting the biopharmaceuticals into the cell and even into specific subcellular compartments like mitochondria. This overview illustrates cellular barriers and current status of non‐viral gene vectors, i.e. , lipoplexes, liposomes, polyplexes, and nanoparticles, to relocate therapeutic DNA‐based nanomedicine into the target cell. Despite the awesome impact of physical methods ( i.e. , ultrasound, electroporation), chemical methods have been shown to accomplish high‐level and safe transgene expression. Further comprehension of barriers and the mechanism of cellular uptake will facilitate development of nucleic acids‐based nanotherapy for alleviation of various disorders.