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Gene targeting from laboratory to livestock: Current status and emerging concepts
Author(s) -
Laible Götz,
AlonsoGonzález Lucía
Publication year - 2009
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200900006
Subject(s) - livestock , current (fluid) , computational biology , microbiology and biotechnology , biology , business , nanotechnology , risk analysis (engineering) , engineering , materials science , ecology , electrical engineering
The development of methods for cell‐mediated transgenesis, based on somatic cell nuclear transfer, provides a tremendous opportunity to shape the genetic make‐up of livestock animals in a much more directed approach than traditional animal breeding and selection schemes. Progress in the site‐directed modulation of livestock genomes is currently limited by the low efficiencies of gene targeting imposed by the low frequency of homologous recombination and limited proliferative capacity of primary somatic cells that are used to produce transgenic animals. Here we review the current state of the art in the field, discuss the crucial aspects of the methodology and provide an overview of emerging approaches to increase the efficiency of gene targeting in somatic cells.