Premium
Rapid and label‐free bacteria detection by surface plasmon resonance (SPR) biosensors
Author(s) -
Dudak Fahriye Ceyda,
Boyacı İsmail Hakkı
Publication year - 2009
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200800316
Subject(s) - biosensor , surface plasmon resonance , analyte , nanotechnology , surface modification , bacteria , materials science , chemistry , nanoparticle , chromatography , biology , genetics
Surface Plasmon Resonance (SPR) biosensor technology has been successfully used for the detection of various analytes such as proteins, drugs, DNA, and microorganisms. SPR‐based immunosensors that coupled with a specific antigen‐antibody reaction, have become a promising tool for the quantification of bacteria as it offers sensitive, specific, rapid, and label‐free detection. In this paper, we review the important issues in the development of SPR‐based immunoassays for bacteria detection, concentrating on instrumentation, surface functionalization, liquid handling, and surface regeneration. In addition, this review touches on the recent advances in SPR biosensing for sensitivity enhancement.