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Modified Cetyltrimethylammonium bromide method improves robustness and versatility: The benchmark for plant RNA extraction
Author(s) -
White Elizabeth J.,
Venter Mauritz,
Hiten Nicholas F.,
Burger Johan T.
Publication year - 2008
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200800207
Subject(s) - robustness (evolution) , bromide , benchmark (surveying) , rna , rna extraction , chemistry , extraction (chemistry) , computer science , biological system , chromatography , biochemical engineering , biology , biochemistry , engineering , organic chemistry , gene , geodesy , geography
A wide range of plant RNA extraction methods are available; however, many of these are limited in their application for a diverse range of plant species. With special emphasis on robustness and versatility, we have improved the cetyltrimethylammonium bromide (CTAB) method and isolated high‐quality RNA from 16 different plant species. The major modifications made to the protocol described here were a reduction of sample treatment steps and an increase in β‐mercaptoethanol concentration (to 3%) resulting in a robust, rapid and reproducible plant RNA extraction protocol that can be used for a broad range of plant species and tissue types.