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High‐throughput protein production – Lessons from scaling up from 10 to 288 recombinant proteins per week
Author(s) -
Tegel Hanna,
Steen Johanna,
Konrad Anna,
Nikdin Hero,
Pettersson Katarina,
Stenvall Maria,
Tourle Samuel,
Wrethagen Ulla,
Xu LanLan,
Yderland Louise,
Uhlén Mathias,
Hober Sophia,
Ottosson Jenny
Publication year - 2009
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200800183
Subject(s) - recombinant dna , proteomics , protein expression , human protein atlas , protein purification , protein biosynthesis , protein engineering , computational biology , chemistry , biology , biochemistry , enzyme , gene
The demand for high‐throughput recombinant protein production has markedly increased with the increased activity in the field of proteomics. Within the Human Protein Atlas project recombinantly produced human protein fragments are used for antibody production. Here we describe how the protein expression and purification protocol has been optimized in the project to allow for handling of nearly 300 different proteins per week. The number of manual handling steps has been significantly reduced (from 18 to 9) and the protein purification has been completely automated.