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Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells
Author(s) -
Mohan Chaya,
Kim YeonGu,
Koo Jane,
Lee Gyun Min
Publication year - 2008
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200700249
Subject(s) - chinese hamster ovary cell , recombinant dna , cell culture , glycoprotein , microbiology and biotechnology , biology , biochemistry , genetics , gene
Recombinant glycoprotein therapeutics have proven to be invaluable pharmaceuticals for the treatment of various diseases. Chinese hamster ovary (CHO) cells are widely used in industry for the production of these proteins. Several strategies for engineering CHO cells for improved protein production have been tried with considerable results. The focus has mainly been to increase the specific productivity and to extend the culture longevity by preventing programmed cell death. These CHO cell engineering strategies, particularly those developed in Korea, are reviewed here.