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Multiplex pathogen detection based on spatially addressable microarrays of barcoded resins
Author(s) -
Blais David R.,
AlvarezPuebla Ramon A.,
BravoVasquez Juan P.,
Fenniri Hicham,
Pezacki John Paul
Publication year - 2008
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200700236
Subject(s) - multiplex , dna microarray , computational biology , nanotechnology , biology , computer science , bioinformatics , materials science , genetics , gene , gene expression
Abstract Suspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high‐throughput multiplex screening. We demonstrate a multiplex assay based on antibody‐functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, Franciscella tularensis lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format.