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Culture of transgenic Drosophila melanogaster Schneider 2 cells in serum‐free media based on TC100 basal medium
Author(s) -
Galesi Adriana L. L.,
Pereira Carlos A.,
Moraes Ângela M.
Publication year - 2007
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200700048
Subject(s) - hydrolysate , glutamine , drosophila melanogaster , cell culture , fetal bovine serum , transfection , bovine serum albumin , emulsion , biochemistry , chemically defined medium , transgene , microcarrier , poloxamer , chemistry , biology , microbiology and biotechnology , cell , in vitro , amino acid , genetics , hydrolysis , gene , organic chemistry , copolymer , polymer
Requirements of eliminating animal proteins from cell culture have intensified in recent years, with the pressure of regulatory agencies related to biopharmaceuticals production. In this work, the substitution of fetal bovine serum by yeastolate and a soy hydrolysate (Hy Soy) for the culture of Drosophila melanogaster Schneider 2 cells transfected for the production of rabies virus G glycoprotein was evaluated. TC100 supplemented with glucose, glutamine, lipid emulsion and Pluronic F68 was employed as basal medium. Results show that yeastolate was more efficient on cell growth stimulation than Hy Soy. Cells adapted in medium formulation supplemented with 3 g/L yeastolate, 1% lipid emulsion, 10 g/L glucose, 3.5 g/L glutamine and 0.1% Pluronic F68 attained a maximum concentration of 10.7 × 10 6 cells/mL, with the expression of 9.4 ng/mL G glycoprotein.