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Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose
Author(s) -
KapplerTanudyaya Nathalie,
Schmitt Heike,
Tippkötter Nils,
Meyer Lina,
Lenzen Sigurd,
Ulber Roland
Publication year - 2007
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200700004
Subject(s) - biotransformation , glucokinase , chromatography , extraction (chemistry) , sugar , chemistry , yield (engineering) , affinity chromatography , enzyme , biochemistry , materials science , metallurgy
Mannoheptulose is a seven‐carbon sugar. It is an inhibitor of glucose‐induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently.

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